Cell viability and proliferation assay
Trypan blue (Sigma-Aldrich) staining was used to test for cell viability. The dye stains dead cells, and livings are distinguished by their ability to exclude the dye performing phase contrast microscopy. Cell viability was calculated using the following formula: Cell viability = unstained cells/unstained + trypan blue stained cells × 100%. Cell proliferation was measured using the Cell Counting Kit-8 (Dojindo Laboratories, Kumamoto, Japan) according to the manufacturer's instructions. In this assay 5,000–8,000 cells/well were plated in a 96-well plate and grown overnight, and then treated for 24 h with gemcitabine or Veramil. Cell proliferation was then determined using a VersaMax tunable microplate reader and Softmaxpro 5.2 software for data analysis (Molecular Devices, Sunnyvale, CA, USA).
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Your heart and the walls of your arteries contain special muscle cells. These muscle cells need calcium to contract. Veramil reduces the amount of calcium that goes into these muscle cells. This causes the muscle cells to relax which widens your arteries and reduces the force and rate of your heartbeat. Opening up your arteries lowers your blood pressure and increases the flow of blood to your heart, which helps ease angina. Because Veramil blocks calcium going into special conducting cells in your heart, it is used to treat certain arrhythmias where the heart rate is abnormally fast.
Veramil has also been found helpful in preventing migraine headaches in some people. This leaflet does not contain information about this, but your doctor can give you more information if you have been prescribed it for this reason.
What happens if I overdose?
Seek emergency medical attention or call the Poison Help line at 1-800-222-1222. An overdose of Veramil can be fatal.
Veramil side effects
Get emergency medical help if you have signs of an allergic reaction: hives; difficult breathing; swelling of your face, lips, tongue, or throat.
Call your doctor at once if you have:
a light-headed feeling, like you might pass out;
shortness of breath (even with mild exertion), swelling, rapid weight gain;
fever, upper stomach pain, not feeling well; or
lung problems--anxiety, sweating, pale skin, wheezing, gasping for breath, cough with foamy mucus.
Common side effects may include:
This is not a complete list of side effects and others may occur. Call your doctor for medical advice about side effects. You may report side effects to FDA at 1-800-FDA-1088.
Blood pressure-lowering drugs
- angiotensin-converting enzyme (ACE) inhibitors such as captopril or lisinopril
- diuretics (water pills)
- beta-blockers such as metoprolol or propranolol
Combining blood pressure-lowering drugs with Veramil can lower your blood pressure to a dangerous level. If your doctor prescribes these drugs with Veramil, they will monitor your blood pressure closely.
Is Veramil safe to take if I'm pregnant or breastfeeding?
Safety of Veramil during pregnancy has not been established. Veramil crosses the placenta and enters the fetus.
Safety in nursing mothers has not been established. Veramil is excreted in human milk.
How should I use Veramil?
Veramil injection is given as an infusion into a vein, usually in an emergency situation. A healthcare provider will give you this injection. Your heart rate will be constantly monitored to help determine when your heartbeats have returned to normal.
Oral Veramil is taken by mouth. Follow all directions on your prescription label. Your doctor may occasionally change your dose. Do not use this medicine in larger or smaller amounts or for longer than recommended.
Swallow a capsule or tablet whole and do not crush, chew, break, or open it.
Your blood pressure will need to be checked often. Your liver function may also need to be checked.
If you need surgery, tell the surgeon ahead of time that you take Veramil.
If you have high blood pressure, keep using this medicine even if you feel well. High blood pressure often has no symptoms. You may need to use blood pressure medicine for the rest of your life.
Store at room temperature away from moisture, heat, and light.